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The Green Monster Verfahren zur Erzeugung von Hefestämmen Buchwert Multiple Deletionen
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The Green Monster Verfahren zur Erzeugung von Hefestämmen Buchwert Multiple Deletionen

Article DOI: 10.3791/4072-v • 13:06 min • December 15th, 2012
December 15th, 2012 •
1, 1, 2, 3, 2, 3,4
1Department of Synthetic Biology and Bioenergy, J. Craig Venter Institute, 2Department of Microbial and Environmental Genomics, J. Craig Venter Institute, 3Donnelly Centre & Department of Molecular Genetics, University of Toronto, 4Lunenfeld Research Institute, Mt Sinai Hospital

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Das Green Monster Verfahren ermöglicht die schnelle Montage mehrerer Deletionen mit einem Reporter-Gen kodiert grün fluoreszierendes Protein markiert. Dieses Verfahren basiert auf der Fahrt Hefestämme durch wiederholte Zyklen von sexueller Sortiment von Deletionen und Fluoreszenz-basierter Anreicherung von Zellen, die mehrere Deletionen basiert.

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Mikrobiologie Genetik Synthetische Biologie Umwelt Genomics Genomics Bioengineering Biomedical Engineering Cellular Biology Multi-Site-genomische Engineering genetische Interaktion grün fluoreszierendes Protein GFP Durchflusszytometrie, Hefe Green Monster
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