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In-vitro-Analyse der PDZ-abhängigen CFTR Makromolekulare Signalkomplexe
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In-vitro-Analyse der PDZ-abhängigen CFTR Makromolekulare Signalkomplexe

Article DOI: 10.3791/4091-v • 10:05 min • August 13th, 2012
August 13th, 2012 •
1, 1, 1,2,3
1Department of Biochemistry & Molecular Biology, Wayne State University School of Medicine, 2Cardiovascular Research Institute, Wayne State University School of Medicine, 3Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine

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Zystische Fibrose-Transmembran-Regulator (CFTR), eine epitheliale Chlorid-Kanal, wurde berichtet, dass mit verschiedenen Proteinen zu interagieren und zu regulieren wichtige zelluläre Prozesse, darunter die CFTR PDZ-Motiv-vermittelte Wechselwirkungen sind gut dokumentiert. Dieses Protokoll beschreibt Methoden, die wir entwickelt, um eine PDZ-abhängigen CFTR makromolekularen Signalkomplexes montieren

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Biochemie Molekulare Biologie Chemie CFTR makromolekularen Komplex Protein-Interaktion PDZ Gerüstprotein Epithelzellen Mukoviszidose
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