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Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
 

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting

Article DOI: 10.3791/4111-v 08:14 min September 25th, 2012
September 25th, 2012

Chapters

Summary

The identification of brain tumor initiating cells (BTICs), the rare cells within a heterogeneous tumor possessing stem cell properties, provides new insights into human brain tumor pathogenesis. We have refined specific culture conditions to enrich for BTICs, and we routinely use flow cytometry to further enrich these populations. Self-renewal assays and transcript analysis by single cell RT-PCR can subsequently be performed on these isolated cells.

Tags

Primary Brain Tumor Tissue Stem Cell Assays Flow Sorting Brain Tumor Initiating Cells (BTICs) Neural Stem Cells (NSCs) Culture Conditions Serum-free Medium BFGF EGF Tumorspheres Cell Surface Markers Flow Cytometry Self-renewal Assays 96 Well Plates Stem/progenitor Cells Limiting Dilution Analysis Single Cell RT-PCR
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