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Fabrication and Use of MicroEnvironment microArrays (MEArrays)
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Fabrication and Use of MicroEnvironment microArrays (MEArrays)

Article DOI: 10.3791/4152-v • 11:57 min • October 11th, 2012
October 11th, 2012 •
1,2, 1, 1
1Life Science Division, Lawrence Berkeley National Laboratory, 2Department of Comparative Biochemistry, University of California, Berkeley

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Summary
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A combinatorial functional screening method for gaining insights into the impacts of the molecular composition of microenvironments on cellular functions is described. The method takes advantage of existing microarray-based technologies to generate arrays of defined combinatorial microenvironments that support cell adhesion and functional analysis.

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Fabrication Use MicroEnvironment MicroArrays MEArrays Interactions Cells Microenvironment Functional Consequences Single Cell Level Differentiation Migration Proliferation Phenotypes Tissue Level Morphogenesis Tumorigenesis Cell-cell Interactions ECM Interactions Soluble Factor Interactions Physical Characteristics Genomic Content Combinatorial Interactions Microenvironmental Components Distinctive Behaviors MEArray Platform Cell-based Functional Screening Molecular Composition Elastic Modulus Microarray Technology Micropatterning Technology
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