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Kwantitatieve, real-time analyse van Base excisie reparatie Activiteit in Cellysaten gebruik te maken van laesie-specifieke moleculaire Beacons

Article DOI: 10.3791/4168-v • 15:01 min • August 6th, 2012
August 6th, 2012 •
1,2, 3, 1,2,4
1Department of Pharmacology & Chemical Biology, University of Pittsburgh School of Medicine, 2Hillman Cancer Center, University of Pittsburgh Cancer Institute, 3Department of Experimental Therapy, The Netherlands Cancer Institute, 4Department of Human Genetics, University of Pittsburgh School of Public Health

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We beschrijven een methode voor de kwantitatieve, real-time meting van DNA glycosylase en AP endonuclease activiteiten in cel nucleaire lysaten. De test levert de tarieven van de DNA-reparatie-activiteit vatbaar zijn voor kinetische analyse en is aanpasbaar voor de kwantificering van DNA Repair activiteit in weefsel en tumor lysaten of met gezuiverde eiwitten.

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Moleculaire Biologie Genetics Cancer Biology Base excisie reparatie DNA glycosylase AP endonuclease TL- real-time activiteit test moleculaire baken biomarker DNA-schade basis laesie
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