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JoVE Journal
Neuroscience

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荧光激活细胞分选(FACS)自主神经祖细胞的分离,从胎鼠的内脏器官的优化程序
 
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荧光激活细胞分选(FACS)自主神经祖细胞的分离,从胎鼠的内脏器官的优化程序

Article DOI: 10.3791/4188-v 12:07 min August 17th, 2012
August 17th, 2012

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Summary

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Click here for the English version.

一个优化的过程,净化神经嵴源性神经祖细胞从小鼠胚胎组织被描述。此方法采用荧光报告基因的表达,通过荧光激活细胞分选(FACS)分离的离散人口。该技术可以应用到隔离整个发展或从成人组织中的神经元亚群。

Tags

66期神经科学,医学,分子生物学荧光激活细胞分选,神经祖细胞,酪氨酸羟化酶,鼠标,解剖,分离,自主神经支配,神经嵴
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