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JoVE Journal Neuroscience
En Optimalisert Prosedyre for fluorescens-aktivert Cell Sorting (FACS) Isolering av Autonome Neural stamfedre fra Visceral organer Fetal Mus
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En Optimalisert Prosedyre for fluorescens-aktivert Cell Sorting (FACS) Isolering av Autonome Neural stamfedre fra Visceral organer Fetal Mus

Article DOI: 10.3791/4188-v • 12:07 min • August 17th, 2012
August 17th, 2012 •
1, 1, 1, 1
1Division of Genetic Medicine, Department of Medicine, Vanderbilt University School of Medicine

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En optimalisert prosedyre å rense neural crest-avledet nevrale stamceller fra foster mus vev er beskrevet. Denne metoden tar nytte av uttrykk fra fluorescerende reporter alleler å isolere diskrete populasjoner av fluorescens-aktivert celle sortering (FACS). Teknikken kan brukes til å isolere nevrale subpopulasjoner gjennom utvikling eller fra voksne vev.

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Neuroscience medisin molekylærbiologi fluorescens Aktivert Cell Sortering neuronal stamfedre tyrosin hydroksylase mus Dissection Dissosiasjon autonome innervasjon Neural Crest
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