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Immunology and Infection

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JoVE Journal Immunology and Infection
A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
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A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Article DOI: 10.3791/4216-v • 13:30 min • November 7th, 2012
November 7th, 2012 •
1, 1
1Institute for Bioscience and Biotechnology Research, University of Maryland

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Summary
Automatic Translation

A novel directed evolution method specific to the field of thermostability engineering was developed and consequently validated for bacteriolytic enzymes. After only one round of random mutagenesis, an evolved bacteriolytic enzyme, PlyC 29C3, displayed greater than twice the residual activity when compared to the wild-type protein after elevated temperature incubation.

Tags

Directed Evolution Thermostable Bacteriolytic Enzymes Screening Method Molecular Engineering Protein Properties Rational-based Approaches Variants Structural Stability Endolysins Bacteriophage Peptidoglycan Cell Wall Lysis PlyC Endolysin PlyCA Subunit PlyCB Subunit Group A Streptococci (GAS)
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