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Journal / Immunology and Infection / Massespektrometrisk Analyse av glykosphingolipid…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Massespektrometrisk Analyse av glykosphingolipid Antigener
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Massespektrometrisk Analyse av glykosphingolipid Antigener

Article DOI: 10.3791/4224-v • 13:09 min • April 16th, 2013
April 16th, 2013 •
1, 2, 3,4
1Undergraduate Program, Rice University, 2Proteomics Facility, Department of Pathology, University of Texas MD Anderson Cancer Center, 3Department of Melanoma Medical Oncology, University of Texas MD Anderson Cancer Center, 4University of Texas Graduate School of Biological Sciences at Houston

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En spesifikk og sensitiv metode for å få innsikt i uttrykket profilen glykosphingolipid antigener i immun organer og celler er beskrevet. Metoden utnytter ion felle massespektrometri tillater trinnvis fragmentering av glykosphingolipid molekyler for strukturell analyse i forhold til kjemisk syntetiserte standarder.

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Immunologi biokjemi molekylærbiologi cellebiologi Structural Biology medisin genetikk proteomikk Proteiner glycomics Funksjonell glycomics glykosfingolipider GSLs antigener natural killer T-celler MALDI-TOF massespektrometri LTQ ion felle masse spektrometer massespektrometri glykolipider lipider
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