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En praktisk og Novel metode til at udvinde Genomisk DNA fra Blood Collection Kits til Plasma Protein Preservation
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En praktisk og Novel metode til at udvinde Genomisk DNA fra Blood Collection Kits til Plasma Protein Preservation

Article DOI: 10.3791/4241-v • 10:44 min • May 18th, 2013
May 18th, 2013 •
1, 1, 1, 1, 1, 2, 1, 1
1Division of Gastroenterology, Hepatology and Nutrition, Department of Pediatrics, Emory University School of Medicine and Children's Health Care of Atlanta, 2Division of Rheumatology, Department of Pediatrics, Emory University School of Medicine and Children's Health Care of Atlanta

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Vi beskriver en ny fremgangsmåde til isolering af genomisk DNA fra fuldblod ved plasma / serologi. Efter plasma kollektion er den komprimerede blodet normalt kasseres. Vores hidtil ukendte fremgangsmåde udgør en betydelig forbedring i forhold til eksisterende metoder og gør DNA og plasma fås fra en enkelt samling, uden at anmode om yderligere blod.

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Genetik Molekylærbiologisk Institut cellebiologi medicin biokemi hæmatologi Proteins Genomics genomisk DNA blodansamlinger P100 rør DNA-ekstraktion buffy coat isolation genotypebestemmelse assays rødt blod fuldblod plasma DNA assay genotypebestemmelse
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