Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
 

An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium

Article DOI: 10.3791/4242-v 06:37 min October 24th, 2012
October 24th, 2012

Chapters

Summary

We describe a live whole animal quantitative measurement for permeability of the embryonic zebrafish brain. The technique analyzes the ability to retain cerebrospinal fluid and molecules of different molecular weights within the neural tube lumen and quantifies their movement out of the ventricles. This method is useful for determining differences in epithelial permeability and maturation during development and disease.

Tags

Permeability Zebrafish Embryonic Neuroepithelium Brain Ventricular System Brain Ventricles Cerebrospinal Fluid (CSF) Protein-rich Fluid Neural Tube Formation Claudin 5a Na,K-ATPase Tight Junction Formation Ventricular Dye Retention Assay Myosin Inhibition
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter