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JoVE Journal Bioengineering
Bacterial Detection & Identification Using Electrochemical Sensors
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Bacterial Detection & Identification Using Electrochemical Sensors

Article DOI: 10.3791/4282-v • 09:30 min • April 23rd, 2013
April 23rd, 2013 •
1,2, 3, 2, 2,4,5
1Research Service, Veterans Affairs Greater Los Angeles Healthcare System, 2Department of Urology, The David Geffen School of Medicine, University of California, Los Angeles, 3GeneFluidics, 4Division of Infectious Diseases, Veterans Affairs Greater Los Angeles Healthcare System, 5Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles

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We describe an electrochemical sensor assay method for rapid bacterial detection and identification. The assay involves a sensor array functionalized with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. Sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase-linked DNA oligonucleotide detector probe produces a measurable amperometric current.

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Bacterial Detection Identification Electrochemical Sensors Blood Glucose Measurement Analyte Detection Signal Transduction Redox Enzyme Amperometric Electrode Sensor Specificity Glucose Sensor DNA Oligonucleotides Sandwich Hybridization Ribosomal RNA (rRNA) Capture Probe Detector Probe Horseradish Peroxidase (HRP) Substrate Oxidation Current Flow
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