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Journal / Immunology and Infection / Plaque-Assay für Murine Norovirus
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Immunology and Infection

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JoVE Journal Immunology and Infection
Plaque-Assay für Murine Norovirus
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Plaque-Assay für Murine Norovirus

Article DOI: 10.3791/4297-v • 10:53 min • August 22nd, 2012
August 22nd, 2012 •
1, 1, 1
1Department of Microbiology and Immunology, University of Michigan, Ann Arbor

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Hier beschreiben wir ein Verfahren, um infektiöse Partikel von murinem Norovirus (MNV), die die einzige Norovirus die effizient repliziert in Zellkultur ist quantifizieren. Die Plaque-Assay nutzt MNV den Tropismus für murinen Makrophagen und können zur Verwendung mit biologischen oder Umweltproben enthaltenden MNV angepasst werden.

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Virology Immunologie Infektion Medizin Mikrobiologie Molekularbiologie Plaque-Assay Norovirus murine Norovirus MNV murine Makrophagen RAW 264.7-Zellen
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