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Övervakning plasmidreplikation i Live däggdjursceller över flera generationer genom fluorescensmikroskopi
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Övervakning plasmidreplikation i Live däggdjursceller över flera generationer genom fluorescensmikroskopi

Article DOI: 10.3791/4305-v • 12:34 min • December 13th, 2012
December 13th, 2012 •
1, 1, 1
1Department of Oncology, University of Wisconsin - Madison

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En metod att observera individuella DNA-molekyler i levande celler beskrivs. Tekniken är baserad på bindningen av en fluorescent märkt lac-repressor-protein till bindningsställen manipulerade i DNA av intresse. Denna metod kan anpassas att följa många rekombinanta DNA i levande celler över tiden.

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Genetik 70 molekylärbiologi Cellulär Biology Genomics DNA-syntes DNA-partitionering plasmider lac-operator lac-repressor däggdjursceller fluorescensmikroskopi
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