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Journal / Medicine / Neo-Islet formazione nel fegato di topi diabetici …
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Neo-Islet formazione nel fegato di topi diabetici da Helper-dipendente adenovirale Vector-Mediated Gene Transfer
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Neo-Islet formazione nel fegato di topi diabetici da Helper-dipendente adenovirale Vector-Mediated Gene Transfer

Article DOI: 10.3791/4321-v • 16:59 min • October 10th, 2012
October 10th, 2012 •
1,2, 1,2,3, 1,2,3
1Department of Medicine, Baylor College of Medicine, 2Division of Diabetes, Endocrinology & Metabolism, Diabetes & Endocrinology Research Center, Baylor College of Medicine, 3Department of Molecular & Cellular Biology, Baylor College of Medicine

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Descriviamo epatica neo-isolotto formazione STZ (streptozotocina)-indotta topi diabetici mediante trasferimento genico di Neurogenin3 (Ngn3) e Betacellulin (Btc) utilizzando helper-dipendente vettore adenovirale (HDAd) e l'inversione di iperglicemia. Il nostro metodo sfrutta le vettori adenovirali dipendenti da helper con la loro alta efficienza nella trasduzione vivo e l'espressione genica lunga durata.

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Medicina Numero 68 Genetica Fisiologia terapia genica Neurogenin3 Betacellulin vettori adenovirali helper-dipendenti il diabete di tipo 1 isolotto neogenesi
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