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Immunology and Infection

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JoVE Journal Immunology and Infection
Flowcytometrisk Isolering af primære murine type II alveolære epitelceller for Funktionelle og molekylære studier
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Flowcytometrisk Isolering af primære murine type II alveolære epitelceller for Funktionelle og molekylære studier

Article DOI: 10.3791/4322-v • 14:48 min • December 26th, 2012
December 26th, 2012 •
1,2, 1,2, 3, 2, 1,2, 1
1Research Group Immune Regulation, Helmholtz Centre for Infection Research, 2Research Group Infection Immunology, Institute of Medical Microbiology, Otto-von-Guericke University, 3Department of Experimental Immunology, Helmholtz Centre for Infection Research

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Vi beskriver den hurtige isolering af primære murine type II alveolære epitelceller (AECII) ved flowcytometrisk negativ selektion. Disse AECII viser høj levedygtighed og renhed og er egnede til en lang række funktionelle og molekylære undersøgelser af deres rolle i respiratoriske tilstande, såsom autoimmune eller infektiøse sygdomme.

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Immunology Cellular Biology molekylærbiologi infektion infektionssygdomme Microbiology alveolær type II epitelceller mus luftveje lunger cellesortering flowcytometri influenza autoimmunitet
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