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Hurtig og effektiv generering af neuroner fra humane pluripotente stamceller i en Multititre Plate Format
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Hurtig og effektiv generering af neuroner fra humane pluripotente stamceller i en Multititre Plate Format

Article DOI: 10.3791/4335-v • 07:27 min • March 5th, 2013
March 5th, 2013 •
1, 1,2, 1
1Max Planck Institute for Molecular Biomedicine, 2Medical Faculty, University of Münster

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Protokoller for neuronal differentiering af pluripotente humane stamceller (hPSCs) er ofte tidskrævende og kræver væsentlige cellekultur færdigheder. Her har vi tilpasset et lille molekyle-differentiering procedure til en multititre pladeformat, hvilket tillader enkel, hurtig og effektiv produktion af humane neuroner på en kontrolleret måde.

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Stem Cell Biology Neuroscience Biomedical Engineering Medicine Bioengineering fysiologi genetik Molekylær Biomedicin menneskelige pluripotente stamceller hPSC neuronal differentiering neuroectoderm embryoid organer kemisk definerede betingelser stamceller neuroner signalveje mid-throughput PCR multititre cellekultur
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