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Journal / Bioengineering / In vitro Synthesis of Native, Fibrous Long…
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Bioengineering

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JoVE Journal Bioengineering
In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen
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In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

Article DOI: 10.3791/4417-v • 07:54 min • September 20th, 2012
September 20th, 2012 •
1,2, 1,2, 1, 1, 1,2
1Department of Chemistry, University of Toronto, 2Institute for Optical Sciences, University of Toronto

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Summary
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Simple and reproducible procedures are described for making three structurally distinct collagen assemblies from a common commercially available Type I collagen monomer. Native type, fibrous long spacing or segmental long spacing collagen can be constructed by varying the conditions to which the 300 nm long and 1.4 nm diameter monomer building block is exposed.

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In Vitro Synthesis Native Collagen Fibrous Long Spacing Collagen Segmental Long Spacing Collagen Extracellular Matrix Structural Scaffolding Mechanical Strength Collagen Fibrils Banding Periodicity Type I Collagen Monomers Hierarchical Assembly Length Scales Microns Commercially Available Collagen Monomer Protocols Mixtures Of Structures Unbanded Fibrils Imaging Atomic Force Microscope
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