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Journal / Immunology and Infection / Peptid: MHC-Tetramer-basierte Anreicherung von…
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Immunology and Infection

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Peptid: MHC-Tetramer-basierte Anreicherung von Epitop-spezifischer T-Zellen

Article DOI: 10.3791/4420-v • 13:58 min • October 22nd, 2012
October 22nd, 2012 •
1, 1
1Center for Immunology and Inflammatory Diseases, and Pulmonary and Critical Care Unit, Massachusetts General Hospital and Harvard Medical School

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Dieses Protokoll beschreibt die Verwendung von Peptid: MHC-Tetrameren und magnetische Mikrobeads zur niederfrequenten Populationen von Epitop-spezifischen T-Zellen zu isolieren und zu analysieren durch Durchflusszytometrie. Dieses Verfahren ermöglicht die direkte Untersuchung von endogenen T-Zell-Populationen von Interesse

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Immunologie Zellbiologie Molekularbiologie T-Zell- T-Zell-Rezeptor Tetramer Durchflusszytometrie antigen-spezifischen Immunologie Immunantwort magnetische Anreicherung,
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