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JoVE Journal
Immunology and Infection

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Peptide: MHC tetrameer-gebaseerde verrijking van epitoop-specifieke T-cellen

Article DOI: 10.3791/4420-v 13:58 min October 22nd, 2012
October 22nd, 2012

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Summary

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Dit protocol beschrijft het gebruik van peptide: MHC tetrameren en magnetische microbolletjes voor laagfrequente populaties van epitoop-specifieke T-cellen isoleren en analyseren door flowcytometrie. Deze methode maakt de directe bestudering van endogene T-celpopulaties plaats van

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Immunology Cellular Biology Molecular Biology T-cel T-cel receptor tetrameer flowcytometrie antigeen-specifieke immunologie immuunrespons magnetische verrijking,
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