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JoVE Journal Bioengineering
Quantitativa FRET (Förster Resonance Energy Transfer) analisi per la determinazione della proteasi SENP1 Cinetica
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Quantitativa FRET (Förster Resonance Energy Transfer) analisi per la determinazione della proteasi SENP1 Cinetica

Article DOI: 10.3791/4430-v • 17:12 min • February 21st, 2013
February 21st, 2013 •
1, 1
1Department of Bioengineering, Bourns College of Engineering, University of California, Riverside

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Un nuovo metodo che utilizza l'analisi quantitativa di FRET (Förster Resonance Energy Transfer) segnali è descritto per lo studio di cinetica enzimatica.

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Bioingegneria Numero 72 Biochimica Biologia Molecolare proteine analisi quantitativa FRET qFRET cinetica enzimatica analisi SENP SUMO plasmide espressione di proteine purificazione di proteine analisi proteasi analisi quantitativa
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