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Selektiv opsamling af 5-hydroxymethylcytosine fra genomisk DNA
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Selektiv opsamling af 5-hydroxymethylcytosine fra genomisk DNA

Article DOI: 10.3791/4441-v • 06:26 min • October 5th, 2012
October 5th, 2012 •
1, 2, 2, 1
1Department of Human Genetics, Emory University School of Medicine, 2Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago

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Beskrives en totrins-mærkning ved anvendelse β-glucosyltransferase (β-GT) for at overføre et azid-glucose til 5-HMC, efterfulgt af klik kemi til at overføre en biotin linker til nem og densitet-uafhængig berigelse. Denne effektive og specifikke mærkning metode gør det muligt berigelse af 5-HMC med ekstremt lav baggrund og højkapacitetsidentifikation epigenomic kortlægning via næste generation sekventering.

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Genetik Chemistry Biofysik 5-Hydroxymethylcytosine kemisk mærkning genomisk DNA high-throughput sekventering
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