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Fremstilling af cellelinier til Betinget Knockdown af genekspression og måling af Knockdown Virkninger på E4orf4-induceret celledød
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Fremstilling af cellelinier til Betinget Knockdown af genekspression og måling af Knockdown Virkninger på E4orf4-induceret celledød

Article DOI: 10.3791/4442-v • 13:54 min • October 21st, 2012
October 21st, 2012 •
1, 1, 1
1Department of Molecular Microbiology, Faculty of Medicine, Technion - Israel Institute of Technology

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Bidrag ACF chromatin remodeling faktor til E4orf4-induceret celledød blev målt. Protokollen omfatter selektion af cellekloner, hvor doxycyclin behandling inducerer betinget knockdown af ACF-underenheder Acf1 og SNF2h, og brugen af ​​DAPI assay til måling af E4orf4-induceret celledød i de inducerbare cellelinjer.

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Genetik Cellular Biology molekylær biologi mikrobiologi Medicin Celledød adenovirus E4orf4 DAPI assay betinget knockdown shRNA
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