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JoVE Journal Neuroscience
Imaging Analyse af Neuron til glia Interaktion i Mikrofluid Kultur Platform (MCP)-baseret Neuronal Axon og glia Co-kultur System
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Imaging Analyse af Neuron til glia Interaktion i Mikrofluid Kultur Platform (MCP)-baseret Neuronal Axon og glia Co-kultur System

Article DOI: 10.3791/4448-v • 09:34 min • October 14th, 2012
October 14th, 2012 •
1, 1,2
1Department of Neuroscience, Tufts University, 2Neuroscience Program, Tufts Sackler School of Graduate Biomedical Sciences

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Denne undersøgelse beskriver procedurerne for at oprette en ny neuronal axon og (astro) glia co-kultur platform. I dette co-kultursystem, bliver manipulation af direkte interaktion mellem en enkelt axon (og enkelt gliacelle) mulig, således mekanistisk analyse af den gensidige neuron til gliale signalering.

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Neuroscience Molecular Biology Cellular Biology Biofysik Microfluidics Mikrofluid kultur platform rumopdelt kultur Neuron til glia signalering neuroner glia cellekultur
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