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Visualisering af endoplasmatiske reticulum lokaliserede mRNA'er i pattedyrceller
Yeast Colony Embedding Method
Journal (Biology)
Yeast Colony Embedding Method
Primer-Free Aptamer Selection Using A Random DNA Library
Journal (Biology)
Primer-Free Aptamer Selection Using A Random DNA Library
 
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Visualisering af endoplasmatiske reticulum lokaliserede mRNA'er i pattedyrceller

Article DOI: 10.3791/50066-v • 10:24 min • December 17th, 2012
December 17th, 2012 •
1, 1
1Department of Biochemistry, University of Toronto

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Her beskriver vi en metode til at synliggøre endoplasmatiske reticulum-associerede mRNA'er i mammale vævskulturceller. Denne teknik involverer selektiv permeabilisering af plasmamembranen med digitonin til fjernelse af cytoplasmatisk indhold efterfulgt af fluorescerende

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Cellular Biology Biokemi Genetik Molecular Biology Genomics mRNA lokalisering RNA digitonin udvinding celle fraktionering endoplasmatiske retikulum sekretion mikroskopi billedbehandling fluorescerende FISH cellebiologi
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