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JoVE Journal
Immunology and Infection

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Real-time Live avbildning av T-cellsignalering Complex Formation
 
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Real-time Live avbildning av T-cellsignalering Complex Formation

Article DOI: 10.3791/50076-v 10:31 min June 23rd, 2013
June 23rd, 2013

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Summary

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Click here for the English version.

Vi beskriver en live-cell imaging metod som ger en inblick i protein dynamik under T-cells aktivering process. Vi visar den kombinerade användningen av T-cell sprider analysen, konfokalmikroskopi och bildanalys för att ge kvantitativa resultat att följa signalering komplexbildning hela T-cell aktivering.

Tags

Immunologi cellbiologi molekylärbiologi medicin T-cells aktivering Live-cell imaging signaltransduktion Konfokalmikroskopi signalering komplex Co-lokalisering analys fluorescens cellbiologi T-cell cell imaging
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