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Kombiniert Immunfluoreszenz und DNA FISH auf 3D erhaltene Interphase Nuclei, um Änderungen in 3D Nuclear Organization Study

Article DOI: 10.3791/50087-v • 13:55 min • February 3rd, 2013
February 3rd, 2013 •
1, 1,2,3,4, 1
1Department of Pathology, New York University School of Medicine, 2New York University Center for Health Informatics and Bioinformatics, 3NYU Cancer Institute, 4Department of Pathology and Yale Cancer Center, Yale University School of Medicine

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Hier beschreiben wir ein Protokoll zur gleichzeitigen Detektion von Histonmodifikationen durch Immunfluoreszenz und DNA-Sequenzen von DNA-FISH durch 3D-Mikroskopie und-Analysen (3D Immuno-DNA FISH) gefolgt.

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Genetik Molekularbiologie Bioinformatik Cancer Biology Pathologie Biomedical Engineering Immunologie Intranukleäre Space Nuclear Matrix Fluoreszenz FISH 3D-DNA FISH DNA Immunfluoreszenz Immun-FISH 3D-Mikroskopie Nuclear Organisation Interphasekernen Chromatin Modifikationen
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