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Udtømning af ribosomalt RNA for Mosquito Gut Metagenomic RNA-seq
Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
Journal (Biology)
Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
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Journal (Biology)
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Udtømning af ribosomalt RNA for Mosquito Gut Metagenomic RNA-seq

Article DOI: 10.3791/50093-v • 06:22 min • April 7th, 2013
April 7th, 2013 •
1, 1, 1
1Department of Biology, New Mexico State University

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En ribosomalt RNA (rRNA) udtømning protokol blev udviklet til at berige messenger RNA (mRNA) for RNA-seq af myg tarmen metatranscriptome. Prøve specifikke rRNA prober, som blev brugt til at fjerne rRNA via subtraktion, blev skabt fra myg og dens tarm mikrober. Udførelsen af ​​protokollen kan resultere i fjernelse af tilnærmelsesvis 90-99% af rRNA.

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Genetik Infektion Infectious Diseases molekylærbiologi cellebiologi mikrobiologi Genomics biologi (generelt) genetik (dyre-og planteliv) biovidenskab Eukaryota bakterier metagenomics metatranscriptome RNA-Seq rRNA udtømning mRNA berigelse myg gut microbiome RNA DNA sekventering
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