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Cryosectioning Gær Fællesskaber for Undersøgelse Fluorescens Patterns
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Cryosectioning Gær Fællesskaber for Undersøgelse Fluorescens Patterns

Article DOI: 10.3791/50101-v • 07:03 min • December 26th, 2012
December 26th, 2012 •
1, 1
1Division of Basic Sciences, Fred Hutchinson Cancer Research Center

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Vi præsenterer en protokol til frysning og cryosectioning gær samfund til at observere interne mønstre af fluorescerende celler. Metoden bygger på methanol-fixing og OLT-indlejring for at bevare den rumlige fordeling af celler uden at inaktivere fluorescerende proteiner inden for et fællesskab.

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Mikrobiologi Molecular Biology Cellular Biology Grundlæggende protokoller Gær, Clinical Laboratory Techniques cytologiske teknikker Environmental Microbiology efterforskningsteknikker Life Sciences cryosectioning sektionering cryotome fastsættelse mikrobielle samfund gærkolonier, Bruger-interaktion
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