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Journal / Biology / Gene Trapping Brug Gal4 i zebrafisk
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Gene Trapping Brug Gal4 i zebrafisk
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Gene Trapping Brug Gal4 i zebrafisk

Article DOI: 10.3791/50113-v • 13:34 min • September 29th, 2013
September 29th, 2013 •
1, 1
1Department of Biology, Temple University

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Denne protokol beskriver metoden til gen fælde insertionsmutagenese hjælp Gal4-VP16 som den primære reporter og GFP / RFP som sekundære journalister i zebrafisk. Omkring én ud af ti højt udtrykker F0 fisk udbytte gen afkom fælde co-udtrykker GFP og RFP. Screeningsproceduren kan let skaleres til at tilpasse størrelsen af ​​det laboratorium, der udfører den insertionsmutagenese skærmen.

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Developmental Biology zebrafisk Mutagenesis Genetik genetik (dyre-og plantearter) Gal4 transposon gen-fælde insertionsmutagenese
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