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Journal / Biology / Gene Trapping Utilizzo Gal4 in Zebrafish
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Biology

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Gene Trapping Utilizzo Gal4 in Zebrafish

Article DOI: 10.3791/50113-v • 13:34 min • September 29th, 2013
September 29th, 2013 •
1, 1
1Department of Biology, Temple University

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Questo protocollo descrive il metodo di trappola gene inserzionale mutagenesi utilizzando Gal4-VP16 come giornalista primario e GFP / RFP come reporter secondarie in zebrafish. Circa uno su dieci-alta esprimendo F0 gene rendimento pesce trappola progenie co-esprimono GFP e RFP. La procedura di screening può essere facilmente scalata per adattarsi alle dimensioni del laboratorio che esegue lo schermo mutagenesi inserzionale.

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Biologia dello Sviluppo Numero 79 Zebrafish mutagenesi genetica genetica (animali e vegetali) Gal4 trasposone trappola gene mutagenesi inserzionale
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