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Generering af stabile humane cellelinjer med tetracyklin-inducerbare (Tet-on) shRNA eller cDNA Expression
 
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Generering af stabile humane cellelinjer med tetracyklin-inducerbare (Tet-on) shRNA eller cDNA Expression

Article DOI: 10.3791/50171-v 09:51 min March 5th, 2013
March 5th, 2013

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

En hurtig og enkel måde at generere humane cellelinier med inducerbare og reversibel cDNA overekspression eller shRNA-medieret knock-down af genet af interesse. Denne metode gør det muligt for forskerne at pålideligt og meget reproducerbart manipulere cellelinier, som er vanskeligt at ændre ved transient transfektion metoder eller konventionelle knockdown / knockout strategier.

Tags

Genetik Medicine Biomedical Engineering Bioengineering cellebiologi molekylærbiologi anatomi fysiologi Pattedyr Proteins Cell Biology vævskultur stabil manipulation af cellelinjer tetracyclin reguleret ekspression cDNA DNA shRNA vektorer tetracyclin promoter ekspression gener kloner cellekultur
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