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Mittels quantitativer real-time PCR zum Donor Cell Engraftment in einem Competitive Murine Bone Marrow Transplantation Modell fest
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Mittels quantitativer real-time PCR zum Donor Cell Engraftment in einem Competitive Murine Bone Marrow Transplantation Modell fest

Article DOI: 10.3791/50193-v • 10:11 min • March 7th, 2013
March 7th, 2013 •
1, 1
1Division of Hematology-oncology, Department of Medicine, Medical University of South Carolina

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Bestimmung Donorzelle Transplantation stellt eine Herausforderung in der Maus Knochenmarktransplantation Modelle, die gut definierte phänotypische Marker fehlen. Wir beschrieben eine Methode zur männlichen Spenders Engraftment bei weiblichen Transplantatempfängers Mäusen zu quantifizieren. Dieses Verfahren kann in allen Mausstämmen zur Untersuchung von HSC-Funktionen verwendet werden.

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Medizin Ausgabe 73 Biomedical Engineering Stammzellbiologie Genetik Immunologie Anatomie Physiologie Chirurgie Y Chromosom hämatopoetische Stammzellen HSC Stammzellen Bone Marrow Transplantation Real-Time Polymerase Chain Reaction rtPCR PCR Chimärismus Y-Chromosom spezifischen Gens Pfropf- Transplantation Isolation Transplantation Zellkultur Mausmodell Tiermodell
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