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In vitro organoide Cultura de tumores de colon primarios Ratón
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In vitro organoide Cultura de tumores de colon primarios Ratón

Article DOI: 10.3791/50210-v • 07:33 min • May 17th, 2013
May 17th, 2013 •
1, 1,2
1Department of Molecular & Integrative Physiology, University of Michigan, 2Department of Internal Medicine, Division of Gastroenterology, University of Michigan

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Un método simple para establecer organoide del tumor de colon murino primaria se describe. Este método utiliza la característica de que las células de tumor de colon sobrevivir y crecer en organoides en medios que contienen factores de crecimiento limitadas, mientras que el colon normal epiteliales no lo hacen.

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Biología del Cáncer Medicina Biología Molecular Biología Celular Ingeniería Biomédica Anatomía Fisiología Genética Oncología Cirugía Organoides células tumorales Neoplasias del Colon cultivadas células primarias Cultura tumor de colon la quelación la colagenasa matrigel organoide EGF cáncer de colon cáncer tumor célula el aislamiento la inmunohistoquímica ratón modelo animal
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