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Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
 

Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens

Article DOI: 10.3791/50231-v 09:44 min January 6th, 2013
January 6th, 2013

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Summary

Laser microdissection is a technique that allows the recovery of selected cells from minute amounts of parenchyma. Here we describe a protocol for acquiring human pancreatic islets from surgical specimens to be used for transcriptomic studies. Our protocol improves the intrinsic autofluorescence of human beta cells, thus facilitating their collection.

Tags

Laser Microdissection Human Pancreatic Islets Surgical Specimens Transcriptomic Studies Proteomic Studies DNA Assessment Chromosomal Analysis RNA Preservation Protocol Target Cells Pancreas Cryostat Glass Slides
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