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Silencing a lungo termine di Intersectin-1s in Polmoni mouse consegna ripetuta di un siRNA specifico tramite liposomi cationici. Valutazione degli effetti Knockdown al microscopio elettronico
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Silencing a lungo termine di Intersectin-1s in Polmoni mouse consegna ripetuta di un siRNA specifico tramite liposomi cationici. Valutazione degli effetti Knockdown al microscopio elettronico

Article DOI: 10.3791/50316-v • 15:55 min • June 21st, 2013
June 21st, 2013 •
*1, *1,2, 1,2
1Department of Pharmacology, Rush University, 2Pulmonary and Critical Care Department, Rush University
* These authors contributed equally

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Ripetute iniezioni retro-orbitali di liposomes/siRNA/ITSN-1s cationici complessi nei topi, ogni 72 ore per 24 giorni, fornire in modo efficiente il duplex siRNA per il mouse microcircolo polmonare riducendo mRNA ITSN-1s e di espressione proteica del 75%. Questa tecnica è altamente riproducibile in un modello animale, non ha effetti collaterali ed evita infortuni mortali.

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Bioingegneria Numero 76 Ingegneria Biomedica Biochimica Genetica Biologia Molecolare Biologia Cellulare anatomia fisiologia medicina immunologia farmacologia modelli animali Malattie Cardiovascolari intersectin-1s siRNA liposomi iniezione retro-orbitale acuta e cronica knockdown ITSN-1s topi transgenici cellule endoteliali liposomi dei tessuti del polmone della perfusione microscopia elettronica modello animale
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