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Journal / Biology / GST-他的净化：一个两步亲和纯化协议屈服全长纯化的蛋白质…
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GST-他的净化：一个两步亲和纯化协议屈服全长纯化的蛋白质
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GST-他的净化：一个两步亲和纯化协议屈服全长纯化的蛋白质

Article DOI: 10.3791/50320-v • 11:22 min • October 29th, 2013
October 29th, 2013 •
*1, *1, *1, 1, 1, 1
1Genome Stability Laboratory, Oncology Axis, Hôtel-Dieu de Québec
* These authors contributed equally

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Summary
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在本协议中，我们展示了一个高效和具有成本效益的小型蛋白质纯化方法，它允许重组蛋白的纯化结合独特的可裂解GST标签和一个小His标签。

Tags

生物化学，80期，遗传学，分子生物学，蛋白质，蛋白质组学，重组蛋白，亲和纯化，谷胱甘肽琼脂糖标签，龙爪金属亲和树脂
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