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JoVE Journal Medicine
En Multiplexed Luciferase-baseret Screening Platform for forhører Kræft-associeret signaltransduktion i dyrkede celler
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En Multiplexed Luciferase-baseret Screening Platform for forhører Kræft-associeret signaltransduktion i dyrkede celler

Article DOI: 10.3791/50369-v • 10:13 min • July 3rd, 2013
July 3rd, 2013 •
1, 1
1Department of Cell Biology, UT Southwestern Medical Center

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Opnå et system level forståelse af cellulære processer er et mål for moderne cellebiologi. Vi beskriver her strategier for multiplexing luciferase journalister af forskellige cellulære funktion endepunkter at afhøre genfunktion hjælp genom-skala RNAi biblioteker.

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Cancer Biology medicin genetik cellebiologi molekylærbiologi biokemi Cancer Biology Bioengineering Genomics Drug Discovery RNA-interferens cellebiologi neoplasmer luciferase reportere functional genomics kemisk biologi high-throughput screening teknologi signaltransduktion PCR transfektion analyse
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