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A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination

Article DOI: 10.3791/50374-v • 12:33 min • June 25th, 2014
June 25th, 2014 •
1, 2, 3, 2
1Department of Nutrional Sciences, University of Wisconsin-Madison, 2Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism, University of Wisconsin-Madison, 3School of Pharmacy, University of Waterloo

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Summary
Automatic Translation

Assaying in vitro β-cell function using isolated mouse islets of Langerhans is an important component in the study of diabetes pathophysiology and therapeutics. While many downstream applications are available, this protocol specifically describes the measurement of intracellular cyclic adenosine monophosphate (cAMP) as an essential parameter determining β-cell function.

Tags

Mouse Pancreatic Islet Isolation Intracellular CAMP Determination Diabetes Mellitus Diabetes Pathophysiology Therapeutic Mechanisms Pancreatic Islets Of Langerhans Insulin Secretion Blood Glucose Concentrations Incretins Cyclic Adenosine Monophosphate (cAMP) Type 2 Diabetes Type 1 Diabetes Disease Progression Therapeutic Interventions
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