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JoVE Journal Neuroscience
Implementazione morsetto dinamico con conduttanze sinaptiche e artificiale nel topo cellule gangliari della retina
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Implementazione morsetto dinamico con conduttanze sinaptiche e artificiale nel topo cellule gangliari della retina

Article DOI: 10.3791/50400-v • 11:46 min • May 16th, 2013
May 16th, 2013 •
1, 2, 3
1Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School and Bosch Institute, University of Sydney, 2The MARCS Institute, University of Western Sydney, 3Discipline of Physiology, School of Medical Sciences, Sydney Medical School and Bosch Institute, University of Sydney

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In questo articolo il video illustra il set-up, le procedure per applicare le patch corpi cellulari e come implementare le registrazioni dinamiche clamp da cellule gangliari in tutto il montaggio del mouse retine. Questa tecnica permette l'indagine del contributo preciso di input sinaptici eccitatori e inibitori, e la loro portata, il tempo di spiking neuronale.

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Neuroscienze Numero 75 Neurobiologia Ingegneria Biomedica Anatomia Fisiologia Biologia Molecolare Biologia Cellulare neuroni neuroni della retina cellule gangliari della retina Occhio Retina Neuroscienze retina le cellule gangliari conduttanza sinaptica conduttanza artificiale tetrodotossina (TTX) di patch clamp morsetto dinamica morsetto conduttanza topo modello animale
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