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Journal / Neuroscience / Mikromanipuleringer af genekspression i Adult…
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Neuroscience

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JoVE Journal Neuroscience
Mikromanipuleringer af genekspression i Adult zebrafisk Brain Brug Cerebroventricular mikroinjektion af Morpholino oligonukleotider
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Mikromanipuleringer af genekspression i Adult zebrafisk Brain Brug Cerebroventricular mikroinjektion af Morpholino oligonukleotider

Article DOI: 10.3791/50415-v • 05:48 min • May 23rd, 2013
May 23rd, 2013 •
1, 1, 1, 1
1DFG-Center for Regenerative Therapies Dresden, Cluster of Excellence (CRTD) and Biotechnology Center (BIOTEC) of the Technische Universität Dresden

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I denne artikel, viser vi en fremgangsmåde til manipulation af genekspression i ventrikulære celler i den voksne zebrafisk telencephalon hjælp antisense morpholino oligonukleotider. Vi præsenterer denne metode som en effektiv og hurtig protokol, der kan anvendes til funktionelle undersøgelser i den voksne hvirveldyr hjernen.

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Neurobiologi Neuroscience Genetik Molekylærbiologisk Institut cellebiologi Developmental Biology biokemi Brain zebrafisk morpholinos Gene Knockdown Techniques morpholinogrupper oligonukleotider cerebroventricular mikroinjektion neurovidenskab radiale gliaceller mikroinjektion genekspression,
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