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Journal / Chemistry / Ongerichte Metabolomics uit biologische…
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Ongerichte Metabolomics uit biologische grondstoffen gebruiken UltraPerformance Liquid Chromatography-hoge resolutie massaspectrometrie (UPLC-HRMS)
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Ongerichte Metabolomics uit biologische grondstoffen gebruiken UltraPerformance Liquid Chromatography-hoge resolutie massaspectrometrie (UPLC-HRMS)

Article DOI: 10.3791/50433-v • 11:00 min • May 20th, 2013
May 20th, 2013 •
1, 1, 1, 1, 1
1Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania

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Ongerichte metabolomics biedt een hypothese genererende momentopname van een metabool profiel. Dit protocol zal de extractie en analyse van metabolieten uit cellen, serum of weefsel tonen. Een reeks van metabolieten worden onderzocht met behulp van vloeistof-vloeistof fase-extractie, microflow UltraPerformance vloeistofchromatografie / hoge-resolutie massaspectrometrie (UPLC-HRMS) gekoppeld aan differentiële analyse software.

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Biochemie Scheikunde Moleculaire Biologie Cellulaire Biologie fysiologie geneeskunde farmacologie Genetica Genomics massaspectrometrie MS Metabolisme metabolomics ongerichte extractie lipiden accurate massa vloeistofchromatografie UltraPerformance vloeistofchromatografie UPLC hoge resolutie massaspectrometrie HRMS spectrometrie
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