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Journal / Chemistry / Vilkårlige Metabolomics fra biologiske kilder…
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JoVE Journal Chemistry
Vilkårlige Metabolomics fra biologiske kilder Bruke UltraPerformance væskekromatografi-High Resolution massespektrometri (UPLC-HRMS)
Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy
Journal (Chemistry)
Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy
Characterizing Lewis Pairs Using Titration Coupled with In Situ Infrared Spectroscopy
Journal (Chemistry)
Characterizing Lewis Pairs Using Titration Coupled with In Situ Infrared Spectroscopy
 
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Vilkårlige Metabolomics fra biologiske kilder Bruke UltraPerformance væskekromatografi-High Resolution massespektrometri (UPLC-HRMS)

Article DOI: 10.3791/50433-v • 11:00 min • May 20th, 2013
May 20th, 2013 •
1, 1, 1, 1, 1
1Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania

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Vilkårlige metabolomikk gir en hypotese generere øyeblikksbilde av en metabolsk profil. Denne protokollen vil demonstrere ekstraksjon og analyse av metabolitter fra celler, serum eller vev. En rekke metabolitter blir kartlagt ved hjelp av væske-væske-fase ekstraksjon, microflow UltraPerformance væskekromatografi / høy-oppløsnings masse-spektrometri (UPLC-HRMS) koblet til differensial-analyse-programvare.

Tags

Biokjemi kjemi molekylærbiologi cellebiologi fysiologi medisin farmakologi genetikk Genomics massespektrometri MS Metabolism Metabolomics vilkårlige utvinning lipider nøyaktig masse væskekromatografi UltraPerformance væskekromatografi UPLC Høy oppløsnings masse-spektrometri HRMS spektrometri
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