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Beredning av Mgm101 rekombinationsprotein av MBP-baserade taggning strategi
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Beredning av Mgm101 rekombinationsprotein av MBP-baserade taggning strategi

Article DOI: 10.3791/50448-v • 11:40 min • June 25th, 2013
June 25th, 2013 •
1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University

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Den jästmitokondriella nucleoid protein, Mgm101, är en Rad52-typ rekombinationsprotein som bildar stora oligomera ringar. Ett protokoll beskrivs för framställning av lösligt rekombinant Mgm101 med maltosbindande protein (MBP)-taggning strategi tillsammans med katjonbyte och storleksuteslutningskromatografi.

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Biokemi 76 genetik molekylärbiologi Proteiner Mgm101 Rad52 mitokondrier rekombination mtDNA maltosbindande protein
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