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Erhvervelse Fluorescens Time-lapse film fra Spirende Gær og analysere encellede Dynamics hjælp poder
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Erhvervelse Fluorescens Time-lapse film fra Spirende Gær og analysere encellede Dynamics hjælp poder

Article DOI: 10.3791/50456-v • 17:01 min • July 18th, 2013
July 18th, 2013 •
1, 1
1Department of Chemical Engineering, Massachusetts Institute of Technology

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Vi præsenterer en simpel protokol til at opnå fluorescensmikroskopi film af voksende gærceller, og en GUI-baseret software pakke til at udtrække encellede tidsseriedata. Analysen omfatter automatiserede afstamning og division tid opgave integreret med visuel inspektion og manuel datasikring sporede data.

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Mikrobiologi cellebiologi molekylærbiologi genetik Biofysik, Mikroskopi fluorescens Cell Biology mikroskopi / fluorescens og time-lapse spirende gær genekspression dynamik segmentering slægt tracking billedet tracking software gær celler billedbehandling
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