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Neuroscience

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JoVE Journal Neuroscience
En Isoleret Semi-intakt Forberedelse af Mouse vestibulære Sensory epitel for Elektrofysiologi og høj opløsning To-foton mikroskopi
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En Isoleret Semi-intakt Forberedelse af Mouse vestibulære Sensory epitel for Elektrofysiologi og høj opløsning To-foton mikroskopi

Article DOI: 10.3791/50471-v • 11:52 min • June 13th, 2013
June 13th, 2013 •
1, 1, 2, 2, 1
1Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School, University of Sydney, 2School of Biomedical Sciences and Pharmacy, University of Newcastle

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Analyse af vestibulære hår celle funktion kompliceres af deres placering dybt inde den sværeste del af kraniet, den petrous tindingebenet. De fleste funktionelle hår celle undersøgelser har brugt akut isolerede hårceller. Her beskriver vi en semi-intakt forberedelse af muse vestibulære epitel for elektrofysiologiske og to-foton mikroskopi studier.

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Neurobiologi Neuroscience cellebiologi molekylærbiologi Biomedical Engineering anatomi fysiologi kirurgi Vestibulær Hårceller epitel to-foton mikroskopi isolerede semi-intakt elektrofysiologi elektroporation mikroskopi væv isolation dyremodel
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