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JoVE Journal Neuroscience
Laser Capture Microdissection av nervceller från Differentierad Human Neuroprogenitor Celler i kultur
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Laser Capture Microdissection av nervceller från Differentierad Human Neuroprogenitor Celler i kultur

Article DOI: 10.3791/50487-v • 12:38 min • September 16th, 2013
September 16th, 2013 •
1,2, 1,2, 1,2
1Section of Endocrinology, Denver VA Medical Center, 2Department of Medicine, University of Colorado Denver School of Medicine

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Mänskliga neuroprogenitor celler (NPC) expanderades enligt prolifererande betingelser. NPC var differentierade i neuron-rika kulturer i närvaro av en kombination av neurotrofiner. Neuronala markörer detekterades genom immunofluorescens-färgning. För att isolera en ren population av nervceller, var NPCs differentieras på PEN-membran diabilder och laser capture microdissection utfördes.

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Neurovetenskap neurobiologi Cellular Biology Celler Odlade nervceller centrala nervsystemet neurodegenerativa sjukdomar mänskliga neuroprogenitor celler neuronal differentiering neuronala markörer astrocyter laser capture microdissection PEN-membran diabilder cellodling
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