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Flow cytometri af bimolekylær Fluorescens Complementation
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Flow cytometri af bimolekylær Fluorescens Complementation: En High Throughput Kvantitativ metode til at studere protein-protein interaktion

Article DOI: 10.3791/50529-v • 11:11 min • August 15th, 2013
August 15th, 2013 •
1, 1
1Department of Pharmacology, University of Illinois at Chicago

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Flowcytometrisk analyse af bimolekylær Fluorescens komplementation giver en høj produktion kvantitativ metode til at studere protein-protein-interaktion. Denne metode kan anvendes til kortlægning proteinbindingssteder og til screening faktorer, der regulerer protein-protein-interaktion.

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Molekylær Biologi Biokemi cellebiologi genetik farmakologi Proteins flowcytometri bimolekylær Fluorescens Complementation BiFC kvantitativ analyse protein-protein-interaktion Förster resonans energi overførsel FRET Bioluminescens Resonance Energy Transfer BRET protein celle transfektion fluorescens mikroskopi
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