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L'analisi citofluorimetrica di bimolecular Complementation Fluorescenza
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L'analisi citofluorimetrica di bimolecular Complementation Fluorescenza: A High Throughput Metodo quantitativo per studiare l'interazione proteina-proteina

Article DOI: 10.3791/50529-v • 11:11 min • August 15th, 2013
August 15th, 2013 •
1, 1
1Department of Pharmacology, University of Illinois at Chicago

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L'analisi citofluorimetrica di bimolecular complementazione fluorescenza fornisce un alto metodo quantitativo di throughput per studiare l'interazione proteina-proteina. Questa metodologia può essere applicata a siti di legame della proteina di mappatura e per lo screening di fattori che regolano l'interazione proteina-proteina.

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Biologia Molecolare Numero 78 Biochimica Biologia Cellulare Genetica Farmacologia Proteine citometria a flusso biomolecolare complementazione fluorescenza analisi interazione proteina-proteina BiFC quantitativa Förster resonance energy transfer FRET bioluminescenza Resonance Energy Transfer Bret proteine cella trasfezione fluorescenza microscopia
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