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Journal / Bioengineering / CometChip: En High-throughput for måling DNA…
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Bioengineering

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CometChip: En High-throughput for måling DNA Damage i Microarrayed menneskeceller 96-Well Platform

Article DOI: 10.3791/50607-v • 10:59 min • October 18th, 2014
October 18th, 2014 •
*1, *2, 3, 1, 1, 2, 2, 1
1Department of Biological Engineering, Massachusetts Institute of Technology, 2Environmental Toxicology, Chulabhorn Graduate Institute, 3Department of Biomedical Engineering, University of Minnesota
* These authors contributed equally

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Vi beskriver her en plattform som gjør kometmetoden påvisning av DNA-skader med enestående gjennomstrømming. Enheten mønstre pattedyrceller i en microarray og muliggjør parallell prosessering av 96 prøver. Tilnærmingen til rette for analyse av grunnleggende nivå for DNA-skade, eksponering-indusert DNA skade og DNA-reparasjonskinetikk.

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Bioteknologi kometmetoden elektroforese microarray DNA-skader DNA-reparasjon
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